Maysaa Ch Al Mohammedawi
Deakin University, Australia
Title: Nanoparticles loaded with 5-Fluorouracil, Leucovorin, and Bovine Lactoferrin, as drug delivery carriers
Biography
Biography: Maysaa Ch Al Mohammedawi
Abstract
Encapsulation of drugs into nanoparticles (NPs) has become a promising approach for improving the efficacy of antitumor drugs. This study evaluates the cytotoxic effect of nanoformulated antitumor drug 5-fluorouracil (5-FU), alone and combined with leucovorin (LV) or iron saturated bovine lactoferrin (Fe-bLf) by, encapsulating into FDA approved biodegradable polymer poly-caprolactone (PCL) NPs. The spherical NPs were 200-300 ± 2.9 nm in size and had drug loading capacities of 90% (5-FU-PCL), 60% (5-FU-LV-PCL) and 80% (5-FU-Fe-bLf-PCL). Drug release of 5-FU and LV after 96 h reached 98.6 % and 99.89%, respectively; Fe-bLf release was 82.28% after 96 h. Both MTT and TUNEL assay results showed that the multi-combinatorial NPs had cytotoxicity as high as 97% and could induce apoptosis in human colon cancer cell lines (Coca-2 and SW480), but had no effect on normal FHs 74 Int cells. These findings highlight the novelty and promise of this drug delivery system, and the results warrant further evaluation in suitable animal model for its future clinical applications.