Biopharmaceutics and Biologic Drugs

Biography

Biography: R.K.Purohit

Abstract

In today’s changing global scenario, ionizing radiation is considered as most potent cause of oxidative stress mediated by free radical flux which induces severe damage at various hierarchical levels in the organization in the living organisms. Testis is a highly prolific tissue with fast cellular renewal and poor antioxidant defense; therefore, it becomes an easy target for the radiation- induced free radicals that have long been suggested as major cause of male infertility. Radiation causes deleterious effects in all forms of life due to increasing utilization and production of modern technology, a simultaneous exposure of organisms to heavy metals is also unavoidable. These heavy metals become toxic when present in large quantities, with increasing the industrial revolution and industrial waste, the emission of lead has increased into the environment. Thus concomitant exposure to lead acetate and ionizing radiation might produce deleterious effect upon biological system. The total environmental burden of toxicants may have greater effect as against their individual impact as expected by their nature. So interaction between radiation and other toxicants represents a field of great potential importance. In the recent years, immense interest has been developed in the field of chemoprotection against radiation and heavy metals induced changes. In view of the potential for practical application, a variety of compounds are being tested for their radioprotective activities. Among these, Emblica holds a great promise. In light of the above, the present study was aimed to evaluate the protective effect of Emblica officinalis extract (ECE) against radiation and lead induced qualitative, quantitative and biochemical alterations in the testes of Swiss albino mice. The animals were exposed to 3.0 and 6.0 Gy of gamma rays with or without lead acetate treatment.. The Emblica was administered seven days prior to irradiation or lead acetate treatment. The animals were divided into seven groups. The non drug treated control groups were administered lead acetate and exposed to irradiation whereas the experimental groups were given Emblica seven days prior to irradiation or lead acetate treatment. Irradiation resulted into significant decrease in the frequency of different spermatogenic cell counts along with severe histo-pathological lesions up to 14th day in control animals and day-14 in experimental animals thereafter, recovery followed towards the normal architecture. ECE pre- treatment effectively prevented radiation induced end of experimentation. Furthermore, ECE administration inhibited radiation and lead induced changes in the testes of mice. These observations signify that the Emblica officinalis extract can be used as an efficient radio- protector against radiation mediated qualitative, quantitative and biochemical alterations in testes.