Teimuraz Lezhava
Head of the Department of Genetics, Director of Institute of Genetics, Tbilisi State University,Georgia
Title: Epigenetic change of genome by peptide bioregulators in extreme old age
Biography
Biography: Teimuraz Lezhava
Abstract
The object of present investigation is to the study modification of chromosome (total heterochromatin, constitutive and facultative heterochromatin) under the influence of peptide bioregulators (Ala-Glu-Asp-Gly, Ala -Glu-Asp-Pro, Lus-Glu-Asp-Ala and Lys-Glu) and heavy metal in cultured lymphocytes derived from old individuals
Methods: The level of total heterochromatin - identified by the method of differential scanning microcalorimetry; the level of facultative heterochromatin – by the method of sister chromatid exchanges (SCE), level of satellite stalk and C-heterochromatin under the combined effect of bioregulators and Co Cl2 have been studied in lymphocyte cultures from individuals at the age of 80 and over.
Results: The results showed that: 1) epigenetics processes – progressive heterochromatinization of total, constitutive (pericentromeric, telomeric and NOR heterochromatin) and facultative heterochromatin occurred with aging; 2)peptide bioregulators induce deheterochromatinization of chromosomes in old age;3) higher level of SCEs (deheterochromatinization) were registered in precentromeric and telomeric heterochromatin upon combined effect of Co ions and peptide bioregulators.
Conclusions: The proposed genetic mechanism responsible for constitutive and facultative heterochromatin epigenetic change (hetero- and deheterochromatinization pericentromeric and telomeric region) of old age may lead to the development of therapeutic treat.