2^nd International Conference and Expo on Biopharmaceutics and Biologic Drugs September 14-16, 2016 San Antonio, USA

Biography:

Hiroyuki Matsuda has completed his PhD at the age of 29 years from Nihon University. His research field is chemical engineering thermodynamics. He is the associate professor of Nihon University. He has published more than 30 papers in reputed journals.

Abstract:

The object of this study is to search a suitable co-solvent of poorly water-soluble bioactive compounds in natural products on the basis of the solubility measurements. In this study, curcumin (diferuloymethane), the Indian solid gold, the major active component of turmetic, was selected as a model bioactive compound. Curcumin is used as a spice in curry and as a coloring agent in yellow mustards, cosmetics, and pharmaceuticals. It has attracted great interest because of its antioxidant, anti-inflammatory, and potential cancer chemopreventive activities. However, the major problem with curcumin is its extremely low solubility in aquous solution and poor bioavailability. If addition of a suitable co-solvent makes an enhancement of solubilities of curcumin, it would be useful for the development of drug or functional food which an efficient systemic absorption is available. In this work, several β-CD derivatives, e.g., 2-hydroxypropyl- (2-HP-), sulfobutyl ether (SBE-), and methyl- (M-) β-CDs, were investigated as a co-solvent. The solubilities of curcumin in water + CD mixed solvents, and a suitable co-solvent for an enhancement of the solubilities in curcumin was examined. The solubilities of curcumin in water + CD mixed solvents at 298.15 K were determined using high-performance liquid chromatography (HPLC). Enhancement in the solubility of curcumin could be achieved in all β-CD derivatives. Maximum solubilization shows M-β-CD, and follows SBE-β-CD and 2-HP-β-CD. Stability constants kc were evaluated by Takeru Higuchi-Konnors solubility method. The order of the determined stability constants were M-β-CD > SBE-β-CD > 2-HP-β-CD.

Biography:

Matteo Micucci has completed his PhD at the age of 29 years from Bologna University and he carries on his research focused on Medicinal Chemisrty and Nutraceuticals at Department of Pharmacy and Biotechnology, University of Bologna. He spent a three months period, as visiting PhD Student, in the Research Laboratory of Medicinal Chemistry of De Montfort University (DMU), Leicester, UK. He has published 19 papers in reputed journals and he is Science Adviser, in the field of Nutraceuticals, Alternative and Complementary Medicines, at Segreteria Particolare of a Senator of the Italian Republic, from October 12th, 2015 to date.

Abstract:

Gastro-intestinal infections constitute important emerging and re-emerging infective worldwide diseases. They are mostly endemic and show a heterogeneous aetiology. Most water-borne diseases caused by microorganisms induce diarrhoea and determine about 5 million deaths per year. The research on anti-diarrheal tools should be focused on the evaluation of substances and chemically charachterized phytocomplexes able to affect intestinal motility and to exert a prebiotic action. Several plants, such as Castanea sativa Mill., Sanseviera liberica Gerome & Labroy, have been shown to inhibit gut peristalsis, through several mechanisms. Furthermore, disparate classes of natural compounds including hydrolysable tannins and flavonoids, restore intestinal functionality, affecting different molecular networks influencing each other’s. Acacia catechu Willd extract (ACE) has been used in Indian Traditional Medicine to manage several diseases including diarrhoea and other gastrointestinal ailments. This extract was shown to contain high amounts of flavonoids, in particular flavan-3-ols. Furthermore, in vitro biological assays were exerted, using tissues from guinea pigs, to assess ACE effects towards induced and spontaneous intestinal smooth muscle contractility. The results demonstrated that ACE reducecs spontaneous and induced colon and ileal smooth muscle contractility via inhibiting muscarinic and histaminergic receptors. Also ACE effects against several pathogenic and non pathogenic bacteria were tested, showing a selective antibacterial activity towards pathogenic strains including, Staphylococcus aureus, Gram-negative Escherichia coli, Salmonella spp, Campilobacter, without inhibiting. These findings suggest that Acacia may represent a nutraceutical option to manage diarrheal infectious and non infectious disesases

Biography:

working as Associate professor of Pharmaceutics, College of Pharmaceutical Sciences , Govt Medical College, Thiruvananthapuram, Kerala, India. 28 years of teaching and research experience. Regarding Colon targeting two articles were published in the international journals (one in press). Formulation and in vitro evaluation of Budesonide microspheres for colon targeting vol 7, issue 2, February 2016.IJPSR. In vivo evaluation of Budesonide microspheres for colon specific drug delivery. Vol 8, issue 4, April 2016 IJPPS.

Abstract:

A number of colonic diseases could be treated more efficiently by delivering the drug locally in the colon. For the management of active, mild to moderate ulcerative colitis, an oral preparation of topical corticosteroid with reduced systemic exposure is preferred. Budesonide, a synthetic non-halogenated potent corticosteroid with high topical anti-inflammatory effect was selected as model drug.The polymer selected; chitosan is biocompatible, non-toxic and can be biodegraded by the microflora present in the human colon. Budesonide microspheres were prepared by ionotropic gelation process and were coated with eudragit S-100 polymer to prevent drug release in the stomach. The effect of concentration of polymer chitosan and cross linking agent TPP on drug entrapment efficiency, particle size and release profile were investigated. To assess the biodegradability of chitosan by the colonic microorganism, in vitro release studies in presence of rat caecal content were also performed. In vivo therapeutic experiment was done using TNBS induced colitis in rats. The therapeutic effect was assessed by determining the damage score, clinical activity score, C/B weight ratio and MPO activity measurement. The study showed that oral administration of budesonide microspheres exerted an affirmative impact on the colonic ulcer healing by decreasing the area of ulceration, reducing the mass of colon by improving the symptoms of colitis. The designed system was highly promising for potential targeting of budesonide to the colonic region with inconsiderable drug release under simulated gastric condition.

Biography:

Kissi Mudie has completed his MSc in medical biochemistry from Addis Ababa University, School of Medicine. He is the director of National clinical chemistry laboratory, Ethiopian Public Health Institute. He has published more than 14 papers in reputed journals and has been serving as an associate researcher.

Abstract:

The safe use of medicines is a critical issue for all health care professionals, Background: Cancer refers to a group of diseases that are associated with a disturbance in the control of cell growth and metabolism. Indeed, the unbalanced control of cellular proliferation is a primary characteristic of cancer cells and, as such, any molecule capable of inhibiting cancer cell proliferation may also be useful as a potential chemo-preventive agent. Throughout history, antioxidants have been the most significant source of anticancer and chemopreventing agents. More than 1,000 different phytochemicals are already proved to possess interesting chemopreventing activities. Antioxidants consist of a wide variety of biologically active phytochemicals including phenolics, flavonoids, carotenoids, etc. that have been shown to suppress early and late stages of carcinogenesis. Objective: To review recent biochemical and molecular mechanisms, in relation to natural and synthetic chemopreventing substances (antioxidants) for cancer control and management. Major findings: Antioxidants exert anticancer effects via a variety of mechanisms, including removal of carcinogenic agents, modulation of cancer cell signaling and cell cycle progression, promotion of apoptosis and modulation of enzymatic activities. Conclusion: This review provides an updated and comprehensive overview on the anticancer effects of antioxidants in-vitro and in-vivo animal models including recent intervention studies. Finally, possible mechanisms of action involving antioxidant and pro-oxidant activity as well as interference with cellular functions are discussed.

Biography:

Dr. R.K.Purohit at present working as Professor of Zoology in Govt. Dungar College, Bikaner (Rajasthan), India. He has 25 five years of teaching and research experience. He has published around 40 research papers in journals of international repute. He has attended dozens of national and International conferences and presented his papers. He has also chaired the sessions in many international conferences. Dr. Purohit has produced 19 Ph.Ds. and 20 M.Phil. scholars under his able supervision. He has visited Singapore, Malaysia and Japan. He is the recipient of many national and international awards. Dr. Purohit has organized one National conference on herbal radioprotection in the year 2004 (October 17=-19, 2004) and an International conference during the January, 2012 (January 24-25, 2012). He is the life member of many academic societies. At present he is holding the prestigious status of National Secretary, Indian Society for Radiation Biology who is specially working in the field of herbal radioprotection.

Abstract:

In today’s changing global scenario, ionizing radiation is considered as most potent cause of oxidative stress mediated by free radical flux which induces severe damage at various hierarchical levels in the organization in the living organisms. Testis is a highly prolific tissue with fast cellular renewal and poor antioxidant defense; therefore, it becomes an easy target for the radiation- induced free radicals that have long been suggested as major cause of male infertility. Radiation causes deleterious effects in all forms of life due to increasing utilization and production of modern technology, a simultaneous exposure of organisms to heavy metals is also unavoidable. These heavy metals become toxic when present in large quantities, with increasing the industrial revolution and industrial waste, the emission of lead has increased into the environment. Thus concomitant exposure to lead acetate and ionizing radiation might produce deleterious effect upon biological system. The total environmental burden of toxicants may have greater effect as against their individual impact as expected by their nature. So interaction between radiation and other toxicants represents a field of great potential importance. In the recent years, immense interest has been developed in the field of chemoprotection against radiation and heavy metals induced changes. In view of the potential for practical application, a variety of compounds are being tested for their radioprotective activities. Among these, Emblica holds a great promise. In light of the above, the present study was aimed to evaluate the protective effect of Emblica officinalis extract (ECE) against radiation and lead induced qualitative, quantitative and biochemical alterations in the testes of Swiss albino mice. The animals were exposed to 3.0 and 6.0 Gy of gamma rays with or without lead acetate treatment.. The Emblica was administered seven days prior to irradiation or lead acetate treatment. The animals were divided into seven groups. The non drug treated control groups were administered lead acetate and exposed to irradiation whereas the experimental groups were given Emblica seven days prior to irradiation or lead acetate treatment. Irradiation resulted into significant decrease in the frequency of different spermatogenic cell counts along with severe histo-pathological lesions up to 14th day in control animals and day-14 in experimental animals thereafter, recovery followed towards the normal architecture. ECE pre- treatment effectively prevented radiation induced end of experimentation. Furthermore, ECE administration inhibited radiation and lead induced changes in the testes of mice. These observations signify that the Emblica officinalis extract can be used as an efficient radio- protector against radiation mediated qualitative, quantitative and biochemical alterations in testes.

Biography:

Jeanne Leblond has completed his PhD at the age of 26 years from Université Paris VI and postdoctoral studies from faculty of Pharmacy of University of Montréal. She is assistant professor at the faculty of pharmacy since 2011. She is the director of the research axis “Drug Formulaiton and Analysis” and has trained over 20 students in 5 years. She has published 13 research articles, 1 book chapter, most of them in journal with IF higher than 4.5.

Abstract:

RNA interference provides a targeted approach for silencing gene expression that may prove beneficial in the treatment of diseases such as cancer and genetic disorders. To ensure effective knockdown, siRNA must be entrapped and efficiently conveyed into the cytoplasm of cells. These hydrophilic nucleic acids have to cross the lipid-rich plasmatic and/or endosomal membrane, without being degraded into lysosomes. We have developed new pH-sensitive lipids able to change conformation upon protonation at endosomal pH values, leading to the disruption of the lipidic bilayer and thus to the fast release of the nucleic acids into the cytosol. The objective of this work was to design a fast-responding system at pH 5 while remaining stable at blood pH value and during storage. This was achieved by the design and synthesis of a series of switchable lipids, and their incorporation into lipid nanoparticle (LNP) composition. LNP complexed with siRNA exhibited high silencing efficiency, reaching up to 10% on HeLa cells, very similar to a commercial agent, with lower toxicity. Negative controls demonstrated that the improved efficiency was due to the conformational switch of the lipids. In vitro transfection potential was confirmed on various cells lines (HeLa, A549, Huh-7) and siRNA targets (GFP, PCSK9, survivin). In vivo applications are currently focused on liver disease, such as hypercholesterolemia. Indeed, liver targeting has been shown in mice by fluorescence imaging. This system has recently been able to reduce the LDL as well as HDL cholesterol blood levels of mice after a single i.v. injection of LNP/siRNA.

Biography:

Jeanne Leblond has completed his PhD from Université Paris VI and Post-doctoral studies from faculty of Pharmacy of University of Montréal. She is Assistant Professor at the Faculty of Pharmacy since 2011. She is the Director of the research axis “Drug Formulation and Analysis” and has trained over 20 students in 5 years. She has published 13 research articles, 1 book chapter, most of them in journal with IF higher than 4.5.

Abstract:

RNA interference provides a targeted approach for silencing gene expression that may prove beneficial in the treatment of diseases such as cancer and genetic disorders. To ensure effective knockdown, siRNA must be entrapped and efficiently conveyed into the cytoplasm of cells. These hydrophilic nucleic acids have to cross the lipid-rich plasmatic and/or endosomal membrane, without being degraded into lysosomes. We have developed new pH-sensitive lipids able to change conformation upon protonation at endosomal pH values, leading to the disruption of the lipidic bilayer and thus to the fast release of the nucleic acids into the cytosol. The objective of this work was to design a fast-responding system at pH 5 while remaining stable at blood pH value and during storage. This was achieved by the design and synthesis of a series of switchable lipids, and their incorporation into lipid nano-particle (LNP) composition. LNP complexed with siRNA exhibited high silencing efficiency, reaching up to 10% on HeLa cells, very similar to a commercial agent, with lower toxicity. Negative controls demonstrated that the improved efficiency was due to the conformational switch of the lipids. In vitro transfection potential was confirmed on various cells lines (HeLa, A549, Huh-7) and siRNA targets (GFP, PCSK9, survivin). In vivo applications are currently focused on liver disease, such as hypercholesterolemia. Indeed, liver targeting has been shown in mice by fluorescence imaging. This system has recently been able to reduce the LDL as well as HDL cholesterol blood levels of mice aft er a single I.V. injection of LNP/siRNA.

Biography:

Professor T. Lezhava's scientific works are generally dedicated to the problem genetics of aging. He has discovered that the progressive heterochromatinization occurs in aging. Professor T. Lezhava is delivering lectures in Genetics, Human Genetics with the Fundamentals of Molecular Genetics, Medicine Genetics and Evolution of Genome.  Professor T. Lezhava is a member of editorial board of Journals:“Georgian medical News”; “Gerontology and Geriatric Research“ (NJ, USA); Jacobs Journal of Gerontology (Texas, USA); Edition “Inter ging” and Journal of Deutscher Wissenschaftsherold” (Germany); Journal of “Biomedicina»(Russia). Professor T. Lezhava is a member of the International Association and USA of Biomedical Gerontology, President of Georgian Gerontology Association,

Head of Georgian Human Genetic Association and member of several local and foreign Scientific Academies.

Abstract:

The object of present investigation is to the study modification of chromosome (total heterochromatin, constitutive and facultative heterochromatin) under  the influence of peptide bioregulators (Ala-Glu-Asp-Gly, Ala -Glu-Asp-Pro, Lus-Glu-Asp-Ala and Lys-Glu) and heavy metal  in cultured lymphocytes derived from old individuals 

Methods: The level of total heterochromatin - identified by the method of differential scanning microcalorimetry;  the level of facultative heterochromatin – by the method of sister chromatid exchanges (SCE), level of satellite stalk and C-heterochromatin   under  the combined effect of  bioregulators  and Co Cl₂ have been studied in lymphocyte cultures from individuals at the age of 80 and over.

Results: The results showed that: 1) epigenetics processes – progressive heterochromatinization of total, constitutive (pericentromeric, telomeric and NOR heterochromatin) and facultative heterochromatin occurred with aging; 2)peptide  bioregulators induce deheterochromatinization of chromosomes in old age;3) higher level of   SCEs (deheterochromatinization) were registered in precentromeric and  telomeric heterochromatin upon  combined effect of Co ions and peptide bioregulators.

Conclusions:  The proposed genetic mechanism responsible for constitutive and  facultative heterochromatin epigenetic change (hetero- and deheterochromatinization pericentromeric and  telomeric region) of old age  may lead to the development of therapeutic treat.

Biography:

Upendra L Patel has completed his PhD from Sardar Patel University, Anand, Gujarat, India in 2010. He is the Head of Department & Associate Professor in Department of Pharmaceutics & Pharmaceutical Technology in Arihant School of Pharmacy & BRI, Gujarat, India. His area of research is formulation and evaluation of controlled drug delivery formulations. He has guided more than 25 MPharm students. He has published more than 40 papers in reputed journals and one book “Dispensing Pharmacy & Drug Store Management”. He is life time member of Gujarat Pharmacy Teacher Associations.

Abstract:

To formulate and evaluate a press coated pulsatile release tablets of prednisolone using an admixture of hydrophilic polymer, i.e., low substituted hydroxy propyl cellulose (L-HPC) and hydrophobic polymer, i.e., ethyl cellulose (Ethocel 10 cps) in order to achieve a pre-determined lag time for chronotherapy of rheumatoid arthritis. Th e press coated pulsatile tablets containing prednisolone in the inner core were prepared by compression coating with L-HPC and Ethocel 10 cps as the outer layer in different ratios. The effect of polymer ratio and weight gain of the outer layer on lag time of drug release was investigated using 32 full factorial design. The parameters determined were tablet hardness, friability, drug content, lag time, in vitro dissolution. The release profile of the press coated tablet exhibited a distinct lag time before burst release of prednisolone. Lag time was dependent on the ratio of L-HPC/Ethocel 10 cps and weight gain in outer shell. The lag time was from 1 to 10 hr and could be modulated as it decreased as the amount of L-HPC in the outer layer increased. A surface plots are also presented to graphically represent the effect of independent variables on the lag time. The validity of generated mathematical model was tested by preparing checkpoint formulation. Formulation PCPT7 with L-HPC/Ethocel 10 cps (10:90) and weight gain 300 mg showing predetermined lag time of 5 hr prior to burst release of the drug from the press coated tablet was taken as the optimized formulation.

Biography:

Negar Taghavi Pourianazar has completed her PhD from Middle East Technical University. She has four published papers. One of her papers which is published in Journal of Nanoparticle Research is a review paper about polymeric nanoparticles called “Bioapplications of Poly(amidoamine) (PAMAM) Dendrimers in Nanomedicine”. Her last paper is published in Biomedicine and Pharmacotherapy with the title of “CpG Oligodeoxynucleotide-loaded PAMAM dendrimer-coated magnetic nanoparticles promote apoptosis in breast cancer cells”. She has presented some of her works as posters and oral presentations in international conferences.

Abstract:

CpG-oligodeoxynucleotide (CpG-ODN) is a potent stimulator of immune responses through triggering of a specific receptor called Toll-like receptor 9 (TLR9). In humans, TLR9 is expressed in numerous cells of the immune system and also in cancer cells. Our previous study indicated that activating of TLR9 by CpG-ODNs can generate a signal cascade for apoptosis in breast cancer cells. The purpose of this study was to examine the effects of CpG-ODN on the expression of the apoptosis-related genes in three different breast cancer cell lines, MDA-MB231, SKBR3, and MCF7. The expression profile of various apoptosis-related genes, Bax, Noxa, Bcl-2, Survivin, PUMA, and C-Flip, was studied and compared between untreated and CpG-loaded DcMNP-treated breast cancer cell lines. In evaluation of expression analysis of Bcl-2 and Bax, ratio of pro-apoptotic Bax to anti-apoptotic Bcl-2 expression was considered as an apoptotic parameter. Bcl-2/Bax ratio increased with CpG-loaded DcMNPs stimulation at the mRNA level in MDA-MB231 cells. Furthermore, a significant enhance in the amount of Noxa mRNA expression has detected in treated MDA-MB231 and MCF7 cells which may contribute to apoptosis in these cell lines. The expression levels of C-Flip and Survivin mRNA were decreased significantly in treated SKBR3 and MDA-MB231 cells, respectively. In conclusion, the mechanism might be that CpG-ODN stimulates cell apoptosis through regulating the expression of apoptosis-related genes. DcMNPs is a targetable efficient delivery system for CpG-ODN which could be considered as a therapeutic agent in breast cancer.

Biography:

Madhuri Sharon has done her Ph.D from Leicester, U.K. and Post Doc from Bolton Institute of Technology, UK. At present she is Director at Walchand Centre of Research in Nanotechnology & Bio-Nanotechnology & Managing Director Monad Nanotech Pvt. Ltd.& Professor Emeritus Adjunct Professor,University of Mumbai.She has published 9 books and 172 articles.

Abstract:

Photoluminescent, Carbon-dots is envisaged as drug delivery vehicle due to their high chemical stability, water solubility, resistance to photo-degradation, biocompatibility, low toxicity and easily tuneable optical properties. Use of natural as well as chemical precursors for synthesis of crystalline C-dots and conjugates of C-dot + mesoporous silica and C-dot + gold nanorod using microwave assisted heating in alkaline environment and their use for delivery of therapeutic payloads is reported here. Sucrose density gradient centrifugation was used to separate C- dots from derbies. Optical and morphological properties of the C-dots were confirmed by UV-Vis spectroscopy, Fluorescence spectroscopy, Raman, XRD and FE-SEM. C-dots were biocompatible against MDCK cells. Drugs studied include ciprofloxacin hydrochloride, Doxorubicin, Haloperidol and Curcumin. C-dots/drug +linker complex were dialyzed against nanopure water to remove unattached drug and characterized by FTIR and TGA. Drug loading efficiency and release kinetics was calculated as per different mathematical models. Cipro@C-dots conjugate enhanced the antimicrobial activity against both model gram positive and negative microorganisms. Bovine Serum Albumin protected C-dots and Doxorubicin complex having Folic Acid (FA) as navigational molecule have shown ‘First Order’ release of drug. Moreover, epifluorescence microscopy of HeLa-cells exhibited bright green fluorescence due to internalization of C-dots specifically targeted with FA in HeLa cells. When C-dots@GNR complex was used for anchoring DOX via covalent and non-covalent pH sensitive chemical bonds it could load 94% drug , that rapidly got released under the influence of near-infrared, thus paving way for an efficient nano-carrier, for controlled drug release as well as for bioimaging; thereby serving as potential tool for theranostics.

Biography:

Lorella Ragni graduated in Chemistry  and Pharmaceutical Science at Bologna University Italy. Soon after the degree she attended the Master in Cosmetic Product Development at Ferrara University Italy.

After a short experience in 1988 as researcher in Synthetic Chemistry at Ancona University Italy, since 1989 she has been working in Angelini covering different positions in the Development Product Department. From 2001 to present   she took over the position of Head of Formulation Department. During her working experience she has been the leader of several scientific groups of formulators and analysts for the development of solid, liquid, semisolid pharmaceutical products and new drug delivery systems. In the last five years she extended her range of activity in the formulation development of Food Supplements, Cosmetics and Medical Device for European Market. She constantly supports the Company in the research and evaluation of new technologies for the feeding of project pipeline and  she was the author of several European and Worldwide Patents.

Abstract:

In the cosmetic field consumers are expecting the latest technology advances to be incorporated into innovative formulations, both in terms of active ingredients (entering the perimeter of cosmeceutics) and in terms of delivery technologies. The recent regulatory framework growing araound the cosmetic world, both in the  EMA and FDA perimeters,  is somewhat changing the way of pursuing innovation in this field. Cosmetics formulators today are looking more and more to mutuate technologies from the pharmaceutical world, looking both at the delivery sisthems and at the new functional ingredients.  This cross-contamination between pharmaceutical and cosmeceutical technologies is very important to promote innovation in this field, and can also maximize the return of the research investment for companies that have a pipeline involving both types of products. However, the challenge is not only to respect regulatory requirements that are constantly evolving in the cosmetic and cosmeceutics world, but also to offer new, smart systems that are able to deliver the active ingredients to one or more skin layers minimizing the systemic adsorption.

Biography:

To be updated

Abstract:

To be updated

Biography:

Livia Valentin has completed her PhD from University of São Paulo School of Medicine- FMUSP and postdoctoral from Harvard Medical School; David Geffen School of Medicine at UCLA; Cleveland Clinic Lerner College of Medicine of Case Werstern University; University of Copenhagen; Utrecht University; Max Planck Institute and Karolinska Institute as a multicenter study. She is the Principal Investigator of the RCT Evaluation of POCD through the MentalPlus® digital game. She has published papers in anesthesia and neuropsychology journals and has been serving as an editorial board member of a indexed journal and reviewer of journal about anesthesiology and neuroscience.

Abstract:

Background: Postoperative cognitive dysfunction (POCD) is a multifactorial adverse event most frequently in elderly patients or people aged over 60 years, neurological and psychiatric diseases. This study evaluated the effect of dexamethasone on POCD incidence after non-cardiac surgery and general anesthesia. Methods: One hundred and forty patients (ASA I–II; age 60–87 years) took part in a prospective randomized study involving the administration or not of 8 mg of IV dexamethasone before deep or superficial anesthesia according to bispectral index. Neuropsychological tests were applied preoperatively and at 3rd, 7th, 21st, 90th and 180th days after surgery and compared with normative data. S100β was evaluated before and 12 hours after induction of anesthesia. Linear regression with inference based on the generalized estimating equations (GEE) method was applied, followed by the post-hoc Bonferroni test considering P<0.05 as significant. Results: On the 3rd postoperative day, POCD was diagnosed in 25.2% of patients receiving dexamethasone plus deep anesthesia, 15.3% of the dexamethasone plus superficial anesthesia group, 68.2% of the deep anesthesia group and 27.2% of the superficial anesthesia group (p<0.0001). Neuropsychological tests showed that dexamethasone plus superficial anesthesia decreased the incidence of POCD, especially memory, attention and executive function. The administration of dexamethasone prevented the postoperative increase in S100β serum levels (p<0.002). Conclusion: Dexamethasone can minimize the incidence of POCD in elderly patients undergoing non-cardiac surgery, especially when associated with superficial anesthesia. The effect of dexamethasone on S100β levels might be related with some degree of neuroprotection.

Biography:

Maysaa Ch Al Mohammedawi has completed her MSc and PhD in Medical Biotechnology from the Department of Biotechnology at Al Nahrain University, Baghdad, Iraq. She became a faculty member and group leader of Medical Biotechnology research over there. She worked on molecular analysis of infectious disease, studies on the virulence factors of the pathogens, and screening anticancer agents among bacterial components. In 2012, she was awarded MoHER (Iraq) career development fellowship. Recently, join the IIE-SRF (US) fellowship at School of Medicine, Deakin University, Australia. Her studies focus on improving the in vitro drug delivery system of antitumor agents toward colon cancer.

Abstract:

Encapsulation of drugs into nanoparticles (NPs) has become a promising approach for improving the efficacy of antitumor drugs. This study evaluates the cytotoxic effect of nanoformulated antitumor drug 5-fluorouracil (5-FU), alone and combined with leucovorin (LV) or iron saturated bovine lactoferrin (Fe-bLf) by, encapsulating into FDA approved biodegradable polymer poly-caprolactone (PCL) NPs. The spherical NPs were 200-300 ± 2.9 nm in size and had drug loading capacities of 90% (5-FU-PCL), 60% (5-FU-LV-PCL) and 80% (5-FU-Fe-bLf-PCL). Drug release of 5-FU and LV after 96 h reached 98.6 % and 99.89%, respectively; Fe-bLf release was 82.28% after 96 h. Both MTT and TUNEL assay results showed that the multi-combinatorial NPs had cytotoxicity as high as 97% and could induce apoptosis in human colon cancer cell lines (Coca-2 and SW480), but had no effect on normal FHs 74 Int cells. These findings highlight the novelty and promise of this drug delivery system, and the results warrant further evaluation in suitable animal model for its future clinical applications.

Biography:

Mohammad-Reza Rouini has completed his PhD from Tehran University of Medical Sciences and Post-doctoral studies from Ottawa University. He is the Director of Biopharmaceutics Lab at Faculty of Pharmacy. He has published more than 100 papers in reputed journals and has been serving as an Editorial Board Member of DARU.

Abstract:

It is a factor Xa inhibitor whose potent anticoagulant and antithrombotic effects have been demonstrated. This study was designed to evaluate the pharmacokinetics of a new generic formulation of rivaroxaban compared with a brand in healthy Iranian volunteers.

Twenty-eight healthy volunteers enrolled in this study. This study employed a randomized, single-dose, two-way crossover method using oral tablets of either Axabin® or Xarelto®, as the test drug and reference drug, respectively. Two tablets (2×10 mg) were administered with 240 ml water. Blood samples were gathered at the following times: 0 hour (predose), and 0.5, 1, 1.5, 2, 2.5, 3, 4,5, 6, 8, 10, 24, and 30 hours after drug administration. To provide an accurate analysis, rivaroxaban plasma concentrations were determined by an UHPLC-MS/MS. The optimum separation was performed with a C18 column using acetonitrile and 10mM ammonium acetate (pH = 3, 70:30, v/v) as a mobile phase, at a flow rate of 0.3 ml/min. Precipitation of proteins was employed for extraction of rivaroxaban from the plasma samples. The quantification range for rivaroxaban was 2.5–600 ng.ml-1. According to FDA guidelines, the bioequivalence assessment’s acceptable range is 80–125% at a 90% confidence interval (CI) for the mean ratios of test/reference formulation of the pharmacokinetic parameters. The 90% CI of parameters were AUC0-30 (82.02–98.31), AUC0-inf (82.4-100.34), and Cmax (82.7–104.49). Therefore, the results proved the claim that the two formulations of rivaroxaban are bioequivalent.

Biography:

Omodamiro O D holds a PhD in Pharmacology from College of Medicine and Health sciences, Abia State University Uturu Abia State Nigeria. He is currently a senior Lecturer in the Pharmacology Unit of Department of Biochemistry, Micheal Okpara University of Agriculture Umudike Abia State Nigeria. He has published over 30 research papers in international reputable journals. His areas of research are cardiovascular/endocrine pharmacology, ethnopharmacology, renal pharmacology and medicinal plants.

Abstract:

Amaranthus viridis Linn. (AV), commonly known as ‘Slender or Green Amaranth’ in English, is a multinational genus of herbs. Several species of are often considered as weeds, as leafy vegetables, cereals and ornamentals. Traditionally, an infusion of the plant (leaves and roots) is used to treat dysentery and epilepsy in children, purify blood, reduce inflammation, hemorrhoids, reduce labor pain and improve appetite in parts of Africa and India. The present study was designed to evaluate the In vitro antioxidant activity and hypolipidemic effect of an ethanol extract of AV leaves (EEAVL) in Wistar albino rats and mice as experimental models. Acute toxicity (LD50) of EEAVL was studied in mice using standard method. Th e result showed a LD50 of 353.6 mg/kg of the extract. Fourteen (14) Wistar albino rats of both sexes were divided randomly into seven (7) groups of two animals, each subjected to different treatment for 14 days. Group A was the non-high fat diet control; Group B was the high fat diet-induced control; Group C received 20 mg/kg of Simvastatin, a standard lipid lowering drug; Group D to G received 250 mg/kg, 125 mg/kg, 62.25 mg/kg and 31.13 mg/kg BW respectively of EEAVL of which all were still maintained on their induced diet. At the end of the treatment, the lipid profile and body weight were estimated and compared with the Simvastatin treated control group. The result showed a significant (p<0.05) decrease in T-Cholesterol, Triglyceride and LDL-C (p>0.05) while HDL-C was increased at the doses studied. EEAVL also caused a decrease in the rate of weight gain. In the in-vitro antioxidant test, there was a concentration dependent increase in the (%) scavenging activity when compared with the Vitamin C (control) for 1, 1-diphenyl-2-picrylhydrazyl (DPPH), Nitric oxide and anti-lipid peroxidation activity of AVL. Th e results showed no significant difference at higher concentrations when compared to the control. On a comparative basis, the measure of the antioxidant eff ectiveness of the extract showed better activity in quenching Nitric oxide radical with IC50 values of 72 mg/ml compared to Lipid peroxidation radicals (78mg/ml) and DPPH radicals (108 mg/ml). The result have shown that Amaranthus viridis L, a traditional folklore medicinal plant has in vitro antioxidant potentials and hypolipidemic effect which may provide therapeutic potentials in the management of Cardiovascular diseases, diabetes and their complications which might be caused by free radical generation and hyperlipidemia.

Biography:

Mega Ehigie born 16th February 1984 maried with 1 kid, Lecturer 1 in the department of pharmaceutical pharm technology obtain his Bachelor of pharmacy Degree (B. pharm )in year 2008 and Doctor of pharmacy (pharm D) and Master degree in pharmaceutic in 2012 all in university of Benin, have 3 publications 2 journal articles

Abstract:

Unlike traditional generic pharmaceuticals, Biosimilars (also known as biopharmaceuticals in the United States) aim to copy a complex recombinant, three dimensional protein structure with high molecular weight small changes in the manufacturing process can alter the poducts effect and safety. According to the guild lines of the European agency for evaluation of medicinal products (EMEA) extensive comparability testing will require to demonstrate that the Biosimilars has a comparable profile in terms of quality, safety and efficacy as the reference products. Various analytical assays are available to compare physiochemicals and biological properties between production batches of a potentially similar biopharmaceuticals (comparability) and in comparison with a reference products (similarity).It is important to recognise the limits of existing assay so that the results can be accurately interpreted for market authorisations. This work examines the quality and limits of such analytical tests to demonstrate comparability and similarity of a Biosimilars products to a reference drug with respect to protein content, activity, physio chemical integrity. Stability, impurities and additives, as well as immunogenicity are discussed. Although several assay are available, reliable tests for safety and efficacy still require development. Furthermore, international standards are missing and materials and methods differ from laboratories making the comparison of results very difficult. Clinical trials and post authorisations pharmacovigilance are essential to quarantee the products safety and efficacy over time. Pharmacovigilance, as part of a comprehensive risk management programme, will need to include regular testing for consistent manufacturing of the drugs.

Biography:

Genet Minale is a lecturer in Ambo University in the department of pharmacy. She received a BSc. in chemistry from school of Natural Science in 2009 and MSc. in Pharmacognosy from school of Pharmacy in 2013 from Addis Ababa University, Ethiopia. In addition to teaching, Genet is one of the researchers on traditional medicine in Ethiopia. She conducts research on antimalarial and antimicrobial activities of some indigenous medicinal plant in Ethiopia and she collaborated on manuscript with her colleagues Dr. Kaleab Asres and Dr. Daniel Bisrat entitled in vitro antimicrobial activities of anthrones from the leaf latex of Aloe sinana in which she presented research compiled by Ethiopian Pharmaceutical Association. She currently founded to conduct a research on indigenous knowledge on traditional medicine and pharmacognostical evaluation of medicinal plant in Ethiopia by collaboration with Wondo Genet Agricultural research center.

Abstract:

Background: Aloe sinana Reynolds is endemic to Ethiopia where its leaf latex is traditionally used in and around the town of Debre Sina and other central highlands of the country for the treatment of various illnesses, including wound, snake bite and malaria. However, despite its use in traditional medicine, to date, there appears to have been no chemical or biological studies published on this plant. Aim: The aim of this study was to investigate the leaf latex of A. sinana for its antibacterial and antifungal activities, and to isolate and characterize the compounds that are responsible for the antimicrobial effect of the latex. Materials and Methods: The latex was extracted with methanol. Isolation of compounds was achieved by repeated preparative TLC, and spectroscopic techniques including 1H, 13C‑NMR and MS were used for characterization of the isolated compounds. Antimicrobial activity tests were performed against 21 bacterial and 4 fungal pathogens using the disc diffusion method. Results and Conclusion: Three compounds isolated from the leaf latex were identified as the anthrones, aloin, aloinoside and microdontin. Among the isolated compounds, aloinoside and microdontin were found to possess comparable activity (MIC 5 μg/ml) with that of ciprofloxacin against several Gram‑negative bacterial strains and Staphylococcus aureus. Additionally, microdontin showed potent and comparable activity with the standard antifungal drug griseofulvin against Penicillum spp. These findings support the traditional uses of the plant for the treatment of various infections and wound.